A role for chondroitin sulphate B in the activity of interleukin 12 in stimulating γ-interferon secretion
- 作者：Garnier ; Pascale ; Gibbs ; Roslyn V. ; Rider ; Christopher C.
- 关键词：Chondroitin sulfate ; Interleukin 12 ; Th1 response ; Natural killer cells ; ChABCase ; chondroitin ABCase ; CS ; chondroitin sulfate ; DMSO ; dimethylsulphoxide ; FGF ; fibroblast growth factor ; GAG ; glycosaminoglycan ; γ ; -IFN ; gamma interferon ; NK ; natural kill
- 刊名：Immunology Letters
- 出版年：2003
- 期刊代码：115_01652478
- 类别：med
- 出版时间：January 2, 2003
- 卷：85
- 期：1
- 页码：53-58
- 文件大小：147 K
摘要
We show, using a murine NK cell line which responds quantitatively to rmIL-12, that treatment with ChABCase, but not other GAGases, results in substantial reductions in the secretion of γ-IFN. Likewise, treatment of the cells with a β-d-xyloside inhibitor of proteoglycan biosynthesis inhibits this cytokine response. In both treatments, the addition of soluble, exogenous GAGs does not relieve the inhibition of γ-IFN secretion. We also demonstrate by ELISA that rmIL-12 binds to CS B. Overall, our studies on this in vitro cellular model of the initiation of Th1 immune responses indicate a major role for cell-surface, iduronate-rich, CS proteoglycan in the biological activity of IL-12.