Synthesis of photolabile transcription initiators and preparation of photocleavable functional RNA by transcription

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• 作者：Faqing Huang ; ^{Faqing.Huang@usm.edu} ; Yongliang Shi
• 关键词：Transcription initiator ; RNA labeling ; RNA biotinylation ; RNA photocleavage ; In vitro selection ; RNA caging
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 July, 2012
• 卷：22
• 期：13
• 页码：4254-4258
• 文件大小：718 K

摘要

Two new photolabile adenosine-containing transcription initiators with terminal thiol and amino functionalities are chemically synthesized. Transcription in the presence of the transcription initiators under the T7 phi2.5 promoter produces 5鈥?thiol- and amino-functionalized RNA conjugated by a photocleavable (PC) linker. Further RNA functionalization with biotin may be achieved through acyl transfer reactions from either biotinyl AMP to the RNA thiol group or biotin NHS to the RNA amino group. Photocleavage of the PC linker displays relatively fast kinetics with a half-life of 4-5 min. The availability of these transcription initiators makes new photolabile RNA accessible for affinity purification of RNA, in vitro selection of functional RNAs, and functional RNA caging.