Peroxidase-like activity and amperometric sensing of hydrogen peroxide by Fe₂O₃ and Prussian Blue-modified Fe₂O₃ nanoparticles

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• 作者：Amit Kumar Dutta^a ; Swarup Kumar Maji^a ; Divesh N. Srivastava^b ; Anup Mondal^a ; Papu Biswas^a ; ^{biswaspapu@rediffmail.com} ; Parimal Paul^b ; ^{ppaul@csmcri.org} ; Bibhutosh Adhikary^a ; ^{bibhutoshadhikary@yahoo.in}
• 关键词：Fe2O3 nanoparticles ; Prussian Blue modified Fe2O3 ; Peroxidase mimic ; Electrocatalytic activity ; Amperometric sensor
• 刊名：Journal of Molecular Catalysis A ; Chemical
• 出版年：2012
• 期刊代码：57_13811169
• 类别：ch
• 出版时间：August, 2012
• 卷：360
• 期：Complete
• 页码：71-77
• 文件大小：1169 K

摘要

Nano-sized crystals of iron oxide (Fe₂O₃) was synthesized from a single-source precursor complex [Fe₃(渭₃-O)(O₂CCH₂Cl)₆(H₂O)₃]NO₃路H₂O by solvothermal process. Afterward it was chemically modified with electro-active Prussian Blue (PB). The resulting Fe₂O₃ and PB-Fe₂O₃ nanoparticles (NPs) were characterized by X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), UV-vis and IR spectroscopic techniques. Structural analyses showed that the average diameter of hexagonal Fe₂O₃ (maghemite) and PB-Fe₂O₃ NPs are 40 and 46 nm, respectively. The as-synthesized nanocomposite (PB-Fe₂O₃ NPs) showed superior catalytic activity toward oxidation of peroxidase substrate 3,3鈥?5,5鈥?tetramethylbenzidine (TMB) in presence of H₂O₂ and follow typical Michaelis-Menten kinetics. Furthermore, the nanocomposite, immobilized on surface of a glassy carbon electrode (GCE), exhibited electrocatalytic activity toward reduction of hydrogen peroxide and can be used for its amperometric detection.