Detection of human rhinovirus C in fecal samples of children with gastroenteritis

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• 作者：Susanna K.P. Lau^a ; ^b ; ^c ; ^d ; ^{skplau@hkucc.hku.hk} ; Cyril C.Y. Yip^d ; David Christopher Lung^e ; Paul Lee^d ; Tak-Lun Que^e ; Yu-Lung Lau^f ; Kwok-Hung Chan^d ; Patrick C.Y. Woo^a ; ^b ; ^c ; ^d ; ^{pcywoo@hkucc.hku.hk} ; Kwok-Yung Yuen^a ; ^b ; ^c ; ^d
• 关键词：Human rhinovirus C ; Gastroenteritis ; Fecal ; Stool ; Children ; Pediatric
• 刊名：Journal of Clinical Virology
• 出版年：2012
• 期刊代码：150_13866532
• 类别：med
• 出版时间：April, 2012
• 卷：53
• 期：4
• 页码：290-296
• 文件大小：691 K

摘要

Background

Despite recent discovery of the novel human rhinovirus species, HRV-C, little is known about the association of HRV-C in diseases other than respiratory tract infections.

Objectives

To investigate the presence of HRV-C in fecal samples of children with gastroenteritis.

Study design

734 fecal samples from hospitalized children with gastroenteritis were subject to picornavirus detection by RT-PCR of the conserved 5鈥?NCR. Positive samples were subject to VP4 and 3D^pol gene analysis for species determination. The clinical and molecular epidemiology of HRV-C and other picornaviruses was analyzed.

Results

Picornaviruses were detected in 113 (15.4%) of 734 fecal samples from children with gastroenteritis by RT-PCR of 5鈥?NCR, with 58 containing potential HRVs and 55 containing other enteroviruses. PCR of the VP4 and 3D^pol regions was positive in 21 and 19 samples respectively (both regions positive in 8 samples). Sequencing analysis showed the presence of HRV-C in four samples, and diverse picornaviruses including HRV-A (n = 2), HEV-A (n = 2), HEV-B (n = 2), HEV-C (n = 21) and HPeV (n = 2) in other samples, with co-detection of HRV-C and HPeV in one sample. Of the four children with HRV-C detected in fecal samples, three presented with diarrhea in the absence of respiratory symptoms, while one also had acute bronchiolitis. The four HRV-C strains from fecal samples belonged to the existing clade of diverse HRV-C genotypes, indistinguishable from previous respiratory strains.

Conclusions

HRV-C can be detected in fecal samples of children with gastroenteritis, in the absence of respiratory symptoms. This study also represented the first to detect HPeV in our population.