Does overfeeding enhance genotype effects on energy metabolism and lipid deposition in breast muscle of ducks?
- 作者：Pascal Chartrin ; Marie-Dominique Bernadet ; Gé ; rard Guy ; Jacques Mourot ; Jean-Franç ; ois Hocquette ; Nicole Rideau ; Michel Jacques Duclos ; Elisabeth Baé ; za
- 关键词：Lipids ; Energy metabolism ; Muscle ; Ducks ; Lipoprotein lipase
- 刊名：Comparative Biochemistry and Physiology - Part A ; Molecular & Integrative Physiology
- 出版年：2006
- 期刊代码：23_10956433
- 类别：abs
- 出版时间：December 2006
- 卷：145
- 期：4
- 页码：413-418
- 文件大小：201 K
摘要
We evaluated the effects of genotype (Muscovy, Pekin and their crossbreed hinny and mule ducks) and feeding levels (overfeeding between 12 and 14 weeks of age vs ad libitum feeding) on energy metabolism and lipid deposition in breast muscle of ducks. Samples of breast muscle (Pectoralis major) were collected at 14 weeks of age from 8 birds per group. Overfeeding induced an accumulation of lipids in breast muscle (1.5- to 1.7-fold, depending on genotype) mainly induced by triglyceride deposition. It also induced a considerable increase in the amounts (expressed as g/100 g of tissue) of saturated and mono-unsaturated fatty acids (SFA, MUFA), while the amounts of poly-unsaturated fatty acids (PUFA) remained unchanged in hinny and Muscovy ducks or slightly increased in Pekin and mule ducks. In breast muscle, overfeeding decreased the activity of the main enzymes involved in lipogenesis from glucose (glucose-6-phosphate dehydrogenase, G6PDH, malic enzyme, ME, acetyl CoA carboxylase, ACX). Lipoprotein lipase (LPL) activity in Pectoralis major muscle was also significantly decreased (− 21%). The ability of muscle tissues to catabolize long-chain fatty acids, as assessed by β-hydroxyacyl CoA dehydrogenase (HAD) activity, was increased in Pectoralis major muscle, as was cytochrome-c oxidase (COX) activity. Hybrid and Pekin ducks exhibited higher levels of ACX and LPL activity in Pectoralis major muscle than Muscovy ducks, suggesting a greater ability to synthesise lipids in situ, and to take up circulating lipids. Total lipid content in breast muscle of hybrid and Pekin ducks was higher than in that of Muscovy ducks. In hybrid and Pekin ducks, lipid composition of breast muscle was characterized by higher amounts of triglycerides, SFA and MUFA than in Muscovy ducks. Finally, oxidative metabolism was greater in Pectoralis major muscles of hybrid and Pekin ducks than in Muscovy ducks, suggesting an adaptative strategy of muscle energy metabolism according to lipid level.