Down-regulation of β1,4GalT V at protein level contributes to arsenic trioxide-induced glioma cell apoptosis

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• 作者：Yuanyan Wei ; Dan Liu ; Yuqing Ge ; Fengbiao Zhou ; Jiejie Xu ; Hong Chen ; Xiaojing Yun ; Jianxin Gu ; Jianhai Jiang
• 关键词：Arsenic trioxide ; Apoptosis ; β ; 1 ; 4-Galactosyltransferase V ; N-glycan
• 刊名：Cancer Letters
• 出版年：2008
• 期刊代码：44_03043835
• 类别：med
• 出版时间：18 August 2008
• 卷：267
• 期：1
• 页码：96-105
• 文件大小：1172 K

摘要

Arsenic trioxide (As₂O₃) has considerable efficacy in treating solid tumors with induction of apoptosis with largely unknown mechanisms. Posttranslational processing of proteins by glycosylation could have multiple regulating roles in the process of apoptosis. Here, we found that the expression of β1,6-linked GlcNAc-bearing N-glycans on cell surface protein was gradually decreased after induction of apoptosis by As₂O₃-treatment. And, As₂O₃ significantly decreased the protein expression level of β1,4GalT V, which effectively galactosylates the β1,6-GlcNAc branch of N-glycans and functions as a positive regulator in glioma development. Furthermore, interfering with the expression of β1,4GalT V in human glioma cell markedly promoted As₂O₃-induced cell apoptosis and β1,4GalT V overexpression significantly reduced As₂O₃-induced glioma cell apoptosis. Taken together, our results suggested that down-regulation of β1,4GalT V expression plays an important role in As₂O₃-induced apoptosis, providing a new mechanism of As₂O₃-induced cell apoptosis and indicating that inhibitors of β1,4GalT V may enhance the therapeutic efficiency of As₂O₃ for malignant glioma.