Cell adhesion and focal contact formation on linear RGD molecular gradients: study of non-linear concentration dependence effects

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• 作者：Anna Lagunas ; PhD^a ; ^b ; ^{alagunas@ibecbarcelona.eu} ; Jordi Comelles ; MSc^a ; ^b ; Elena Martí ; nez ; PhD^a ; ^b ; Elisabet Prats-Alfonso ; PhD^a ; ^d ; Gerardo A. Acosta ; PhD^a ; ^d ; Fernando Albericio ; PhD^a ; ^d ; ^e ; Josep Samitier ; PhD^a ; ^b ; ^c
• 关键词：RGD gradient ; Cell adhesion ; Poly(methyl methacrylate) ; Hydrolysis ; Biotin-streptavidin
• 刊名：Nanomedicine ; Nanotechnology ; Biology and Medicine
• 出版年：2012
• 期刊代码：11_15499634
• 类别：et
• 出版时间：May, 2012
• 卷：8
• 期：4
• 页码：432-439
• 文件大小：854 K

摘要

Cell adhesion onto bioengineered surfaces is affected by a number of variables, including the former substrate derivatization process. In this investigation, we studied the correlation between cell adhesion and cell-adhesive ligand surface concentration and organization due to substrate modification. For this purpose, Arg-Gly-Asp (RGD) gradient surfaces were created on poly(methyl methacrylate) substrates by continuous hydrolysis and were then grafted with biotin-PEG-RGD molecules. Cell culture showed that adhesion behavior changes in a nonlinear way in the narrow range of RGD surface densities assayed (2.8 to 4.4 pmol/cm²), with a threshold value of 4.0 pmol/cm² for successful cell attachment and spreading. This nonlinear dependence may be explained by nonhomogeneous RGD surface distribution at the nanometre scale, conditioned by the stochastic nature of the hydrolysis process. Atomic force microscopy analysis of the gradient surface showed an evolution of surface morphology compatible with this hypothesis.

From the Clinical Editor

The authors observed by AFM nonlinear dependence of cell adhesion on RGD gradient surfaces with different surface densities. The nonlinear characteristics may be explained by non-homogeneous RGD surface distribution at the nanometer scale, conditioned by the stochastic nature of the hydrolysis process.