Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability

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• 作者：Hoang Duc Nguyen ; Quynh Anh Nguyen ; Rita C. Ferreira ; Luis C.S. Ferreira ; Linh Thuoc Tran and Wolfgang Schumann
• 关键词：Promoters lepA ; gsiB ; xylA ; and spac ; htpG
• 刊名：Plasmid
• 出版年：2005
• 期刊代码：124_0147619x
• 类别：abs
• 出版时间：2005
• 卷：54
• 期：3
• 页码：241-248
• 文件大小：333 K

摘要

A series of plasmid-based expression vectors have been constructed allowing stable intracellular expression of recombinant proteins in Bacillus subtilis strains. These expression vectors are based on the recently described Escherichia coli–B. subtilis shuttle vector pMTLBS72 which replicates as theta circles. Besides the weak constitutive promoter P_lepA, we inserted three different controllable promoters: P_gsiB which can be induced by heat and acid shock, and by ethanol, P_xylA and P_spac which respond to the addition of xylose and IPTG, respectively. The versatility of these expression vectors was demonstrated by fusing their promoters to a reporter gene and by overexpression of the HtpG protein with three of them. All recombinant vectors exhibited full structural stability.