Cardiotoxicity associated with sunitinib
- 作者：Peter A Kavsak ; Sebastien Hotte
- 关键词：Cultured epidermal keratinocytes ; Human epidermis ; Fibromodulin ; Small leucine rich proteoglycan
- 刊名：The Lancet
- 出版年：2008
- 期刊代码：182_01406736
- 类别：med
- 出版时间：12 April 2008-18 April 2008
- 卷：371
- 期：9620
- 页码：1244
- 文件大小：41 K
摘要
Fibromodulin is a small leucine-rich proteoglycan that has a central role in the maintenance of collagen fibrils structure, and in regulation of TGF-β biological activity. Although, it is mainly found in cartilage and tendon, little is known regarding the expression of the fibromodulin gene in other cell types. By RT-PCR, real time PCR and immunohistochemistry, we describe the expression of the fibromodulin gene and the presence of the protein in human epidermal keratinocytes (HEK), both in culture and in normal human epidermis. Our results show, for the first time, that fibromodulin gene is constantly expressed in HEK during culture time. Immunostaining showed that fibromodulin is located intracytoplasmically in basal and stratified keratinocytes of the growing colonies, confluent cultures, and epidermis in vivo. The expression and intracellular localization of fibromodulin in HEK is a new finding and opens new possible biological roles for the SLRP family.