Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei

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• 作者：Johanna L. Hö ; ö ; g^a ; ^b ; ^{hoog@colorado.edu} ; ^{johanna.hoog@path.ox.ac.uk} ; Cé ; dric Bouchet-Marquis^a ; J. Richard McIntosh^a ; Andreas Hoenger^a ; Keith Gull^b
• 关键词：Flagellum ; Trypanosoma brucei ; Kinetoplastid ; Paraflagellar rod ; Cryo-electron tomography ; Frozen hydrated sections
• 刊名：Journal of Structural Biology
• 出版年：2012
• 期刊代码：92_10478477
• 类别：ph
• 出版时间：May, 2012
• 卷：178
• 期：2
• 页码：189-198
• 文件大小：3311 K

摘要

Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9 + 2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal.