Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects

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• 作者：Yafei Deng^a ; Kai He^b ; Xiaoli Ye^b ; Xin Chen^a ; Jing Huang^a ; Xuegang Li^a ; ¹ ; Lujiang Yuan^a ; ^{yuanlujiang@hotmail.com} ; Yalan Jin^a ; Qing Jin^a ; Panpan Li^a
• 关键词：AUC ; areas under the curve ; CAT ; catalase ; DM ; diabetes mellitus ; DMSO ; dimethyl sulfoxide ; FBG ; fasting blood glucose ; FBS ; fetal bovine serum ; GFR ; glomerular filtration rate ; MDA ; malondialdehyde ; MTT ; methylthiontrazole ; OGTT ; oral glucose tolerance t
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：7 May, 2012
• 卷：141
• 期：1
• 页码：228-233
• 文件大小：371 K

摘要

Aims

The root of Polygonatum odoratum (YuZhu), also a medicinal food has long been used for the treatment of diabetes. The objective of the study was to characterize the anti-diabetic active fractions or compounds in this herb.

Materials and methods

Fractions with a different polarity were prepared by solvent extraction and macroporous absorptive resin (D101) column and their anti-diabetic potentials were evaluated by glucose uptake in HepG2 cells and STZ-induced diabetic rats. In addition, 伪-glycosidase inhibitory activities of active fractions were measured in vitro and chemical compositions including saponin, total flavonoids and total sugar in the fractions were determined.

Results

The n-buthanol fraction, a saponin-rich fraction obtained by partitioning the ethanol extract with n-buthanol after petroleum ether and acetic ether showed the highest anti-diabetic potential in glucose uptake in HepG2 cells followed by acetic ether fraction which was rich in flavonoids. Further fractionation the saponin-rich fraction using macroporous resin column (D101), polysaccharide, flavonoid and saponin rich fractions were obtained by elution with water, 40%and 60%ethanol, respectively and their anti-diabetic potentials proved by glucose uptake test in HepG2 cells and STZ-induced diabetic rats were in the order of saponin rich fraction > flavonoid rich fraction > polysaccharide rich fraction. Long-term therapy test (60 d) in severe diabetic rats indicated that saponin-rich fraction significantly ameliorated clinical symptoms of diabetes including the elevated blood glucose, body weight loss as well as the increased food and water intake while flavonoid-rich fraction was more potential than saponin-rich fraction to increase superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) level in rat plasma. Additionally, saponin-rich fraction and flavonoid-rich fraction showed 伪-glycosidase inhibitory activity with IC₅₀ value of 2.05 卤 0.32 and 3.92 卤 0.65 mg/ml, respectively.

Conclusion

The results suggested that saponin in this herb was more important than flavonoid in exhibiting anti-diabetic activity and flavonoid contributed more to anti-oxidant activity in vivo.