Synthesis, characterization, and cytotoxicity in vitro of the complex [Mn (Hptc) (phen) (OH)] n

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• 作者：MingChang Zhu ; WenXuan He ; EnJun Gao ; ^{ejgao@yahoo.com.cn} ; Lin Lin ; Ying Zhang ; Lei Dai ; RenShu Wang ; Bo Wang ; MeiLin Wang
• 关键词：HeLa and KB cells ; Fluorescence spectrum ; Gel electrophoresis ; Apoptosis ; Cytotoxicity
• 刊名：Life Sciences
• 出版年：2012
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：9 April, 2012
• 卷：90
• 期：13-14
• 页码：519-524
• 文件大小：811 K

摘要

Aims

A bridging ligand 2,4,6-pyridine tricarboxylic acid (H₃ptc) and its manganese(II) complex [Mn(Hptc)(phen)(OH)]n(Hptc = 2,4,6-pyridine tricarboxylic acid, phen = 1,10-phenanthroline) have been synthesized and characterized.

Main methods

The interaction with DNA (HeLa and KB) was carried out by fluorescence spectrum and gel electrophoresis assay. In vitro apoptosis assay and cytotoxicity assay detect the manganese (II) complex interaction with cancer cells.

Key findings

Fluorescence spectrum demonstrated the ability of the complexes to interact with DNA in an intercalative mode. Gel electrophoresis assay exhibited more effective DNA-cleavage activity. In vitro apoptosis assay of the complexes were examined on HeLa and KB cells, exhibited cytotoxic specificity and a significant cancer cell inhibitory rate.

Significance

The complex may be a latent antitumor agent as a result of its unique interaction mode with DNA and cancer cells inhibition effect.