Surfaced-enhanced cellular fluorescence imaging

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• 作者：Qi Hao^a ; Teng Qiu^a ; ^b ; ^{tqiu@seu.edu.cn} ; Paul K. Chu^b ; ^{paul.chu@cityu.edu.hk}
• 关键词：Surface-enhanced spectroscopy ; Surface plasmons ; Cellular fluorescence imaging ; Fluorescence microscopy ; Plasmonic nanostructures
• 刊名：Progress in Surface Science
• 出版年：2012
• 期刊代码：93_00796816
• 类别：ms
• 出版时间：January-April, 2012
• 卷：87
• 期：1-4
• 页码：23-45
• 文件大小：1401 K

摘要

The novel and burgeoning technique of surfaced-enhanced cellular fluorescence imaging has tremendous potential in the monitoring and investigation of intracellular processes at the single-molecular level, for instance, high-resolution cellular imaging, long-term in vivo observation of cell trafficking, tumor targeting, and diagnostics. The success hinges on the development and fabrication of plasmonic nanostructured surfaces with size and shape compatible with cell interactions because they are crucial to enhanced cellular imaging. In this review, the mechanism of surface-enhanced cellular fluorescence imaging is discussed in view of metal-enhanced fluorescence. The design of nanostructured surfaces with evenly distributed plasmonic fields suitable for enhanced cellular fluorescence imaging such as nanoparticle superlattice coatings, lithographically-based substrates, and alumina-templated surface are described.