Fluorescence study of drug-carrier interactions in CTAB/PBS buffer model systems
- 作者：Anne Techen Anne.Techen@uni-potsdam.de ; Carsten Hille hille@uni-potsdam.de ; Carsten Dosche dosche@uni-potsdam.de ; Michael U. Kumke ; Kumke@uni-potsdam.de
- 关键词：Hexadecyltrimethylammonium bromide ; 5-Hexadecanoylaminofluorescein ; 2 ; 10-Bis-(3-aminopropyloxy)dibenzo[a ; j]perylene-8 ; 16-dione ; Fluorescence correlation spectroscopy ; Fluorescence anisotropy ; Single-molecule FRET
- 刊名：Journal of Colloid and Interface Science
- 出版年：2012
- 期刊代码：125_00219797
- 类别：ch
- 出版时间：1 July, 2012
- 卷：377
- 期：1
- 页码：251-261
- 文件大小：795 K
摘要
The well-known cationic surfactant hexadecyltrimethylammonium bromide (CTAB) was used as a model carrier to study drug-carrier interactions with fluorescence probes (5-hexadecanoylaminofluorescein (HAF) and 2,10-bis-(3-aminopropyloxy)dibenzo[a,j]perylene-8,16-dione (NIR 628) by applying ensemble as well as single molecule fluorescence techniques. The impact of the probes on the micelle parameters (critical micelle concentration, average aggregation number, hydrodynamic radius) was investigated under physiological conditions. In the presence of additional electrolytes, such as buffer, the critical micelle concentration decreased by a factor of about 10. In contrast, no influence of the probes on the critical micelle concentration and on average aggregation number was observed. The results show that HAF does not affect the characteristics of CTAB micelles. Analyzing fluorescence correlation spectroscopy data and time-resolved anisotropy decays in terms of the 鈥渢wo-step鈥?in combination with the 鈥渨obbling-in-cone鈥?model, it was proven that HAF and NIR 628 are differently associated with the micelles. Based on ensemble and single molecule fluorescence experiments, intra- and intermicellar energy transfer process between the two dyes were probed and characterized.