Emergence of antigenic variants with in serotype A foot and mouth disease virus in India and evaluation of a new vaccine candidate panel

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• 作者：Ajjaiah G. Rudreshappa^a ; Aniket Sanyal^a ; Jajati K. Mohapatra^a ; Saravanan Subramaniam^a ; Ankan De^a ; Biswajit Das^a ; Nagendrakumar B. Singanallur^b ; Anil K. Jangam^b ; Madhanmohan Muthukrishnan^b ; Srinivasan A. Villuppanoor^b ; Bramhadev Pattnaik^a ; ^{pattnaikb@gmail.com}
• 关键词：FMDV serotype A ; Genotype 18 ; VP359-deletion group ; Vaccine strain selection
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：17 August, 2012
• 卷：158
• 期：3-4
• 页码：405-409
• 文件大小：330 K

摘要

Emergence of genetically and antigenically divergent lineages/genotypes and poor intergenotypic antigenic coverage is a major concern in serotype A foot-and-mouth-disease virus (FMDV) in India. In 2009, to cover antigenic diversity emerged in serotype A virus field isolates, IND40/2000 was selected as the new vaccine strain for incorporation in the trivalent FMD vaccine formulation used in India. Although current vaccine strain (IND40/2000) covers most isolates antigenically, a few VP3⁵⁹-deletion group isolates showed low r-value in routine vaccine matching exercise. The VP3⁵⁹-deletion group within genotype 18 emerged first in late part of 2002 and in 2007 causing outbreaks along with non-deletion isolates of the same genotype. In case of emergence or re-emergence of more antigenically divergent isolates in future, a need for a new vaccine candidate to cover maximum isolates of both deletion and non-deletion group may arise. Four alternate candidate vaccine strains (IND281/2003, IND195/2007, IND360/2007 and IND123/2008) were selected based on set criteria and antigenic relationships with field isolates sampled between 2002 and 2009 were analyzed using a micro-neutralization test. Phylogenetic analysis based on capsid region of serotype A isolates revealed existence of two broad distinct clusters (VP3⁵⁹-deletion and non-deletion group) within genotype 18. The VP3⁵⁹-deletion group has diversified genetically with time giving rise to three different sub-lineages (clade18a, 18b and 18c). The present study indicates that the virus candidates IND281/2003 (VP3⁵⁹-deletion group) and IND195/2007 (non-deletion group) can be used as an adjunct or alternative strain to currently used vaccine strain IND40/2000 in case of emergence of more antigenically divergent isolates in future.