Black yeast-like fungi associated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae)

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• 作者：Vania A. Vicente^a ; ^b ; R. Oré ; lis-Ribeiro^b ; ^c ; ^d ; M.J. Najafzadeh^a ; ^e ; Jiufeng Sun^f ; Raquel Schier Guerra^b ; ^d ; Stephanie Miesch^a ; Antonio Ostrensky^d ; Jacques F. Meis^g ; Corné ; H. Klaassen^g ; G.S. de Hoog^a ; ^h ; ⁱ ; ^j ; ^{de.hoog@cbs.knaw.nl} ; ^{s.hoog@cbs.knaw.nl} ; Walter A. Boeger^c ; ^d ; ^{wboeger@ufpr.br}
• 关键词：Ucides cordatus ; Mangrove crab ; Lethargic Crab Disease (LCD) ; Exophiala cancerae ; Fonsecaea brasiliensis ; Virulence
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：6 July, 2012
• 卷：158
• 期：1-2
• 页码：109-122
• 文件大小：1732 K

摘要

Lethargic Crab Disease (LCD) caused extensive epizootic mortality of the mangrove land crab m>Ucides cordatusm> (m>Brachyura: Ocypodidaem>) along the Brazilian coast, mainly in the Northeastern region. The disease was named after the symptoms of slow movement of infected crabs. Causative agents were suspected to be two black yeast-like fungi of the family m>Herpotrichiellaceaem> (ascomycete order m>Chaetothyrialesm>), judged by infected tissue biopsies from moribund m>U. cordatusm>. The aim of the present study is to prove that two species are involved in the disease: the recently described black yeast m>Exophiala canceraem>, but also a less virulent, hitherto undescribed fonsecaea-like species, introduced here as the novel species m>Fonsecaea brasiliensism>. Strains were identified by ITS rDNA sequencing, and species borderlines were established by multilocus sequencing and AFLP analysis. m>Fonsecaea brasiliensism> proved to be closely related to the pathogenic species m>Cladophialophora devriesiim> which originally was isolated from a systemic infection in a human patient. The virulence of m>F. brasiliensism> is lower than that of m>E. canceraem>, as established by artificial inoculation of mangrove crabs.