Selective quantification of human DNA by real-time PCR of FOXP2

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• 作者：Mikiko Soejima^a ; Kenichi Hiroshige^a ; ^b ; Joji Yoshimoto^a ; Yoshiro Koda^a ; ^{ykoda@med.kurume-u.ac.jp}
• 关键词：Real-time PCR ; Human identification ; Quantification of DNA ; TaqMan
• 刊名：Forensic Science International ; Genetics
• 出版年：2012
• 期刊代码：18_18724973
• 类别：bio
• 出版时间：July, 2012
• 卷：6
• 期：4
• 页码：447-451
• 文件大小：385 K

摘要

We established a simple quantitative PCR procedure with high specificity and sensitivity using TaqMan probes targeting the FOXP2 sequence. This assay distinguished human and nonhuman, including primates, samples with the exception of mouse, turtle, lizard, and fishes. However, the specific amplification of mouse, lizard, and turtle fragments of FOXP2 could be confirmed by electrophoresis after real-time PCR. Because the C_T values obtained for human DNA were not affected by contaminating animal DNA at concentrations up to 30 times that of human DNA, we were able to estimate the concentration of human DNA in mixed specimens. This assay provides a reliable and useful method for routine quantification of human-specific DNA in forensic practice.