Extraction of proteins from pennycress seeds and press cake

详细信息	查看全文 | 推荐本文 |

• 作者：Gordon W. Selling^a ; ^{gordon.selling@ars.usda.gov} ; Mila P. Hojilla-Evangelista^a ; Roque L. Evangelista^b ; Terry Isbell^b ; Neil Price^c ; Kenneth M. Doll^b
• 关键词：Pennycress ; Protein extraction ; Biodiesel ; Calorimetry
• 刊名：Industrial Crops and Products
• 出版年：2013
• 期刊代码：56_09266690
• 类别：abs
• 出版时间：January, 2013
• 卷：41
• 期：Complete
• 页码：113-119
• 文件大小：549 K

摘要

In order to more fully utilize pennycress, a potentially viable bio-diesel source, the proteinaceous components were extracted from pennycress seeds and press cake. The amino acid composition of the proteins present in pennycress was typical for proteins derived from plants, with glycine, glutamic acid and alanine being prevalent. Water, 0.5 M sodium chloride, 60%acetic acid, 0.1 M sodium hydroxide and ethanol were used in sequential order to remove the protein from pennycress seeds and press cake and determine the various soluble protein fractions. Extraction temperature was varied from 5 to 77 掳C. The highest yield of material (35%) was obtained by extracting pennycress seeds with water at 77 掳C. However, this material had only moderate levels of protein (25%) with the remainder being carbohydrates and oil (as determined by infrared spectroscopy). The use of 0.5 M sodium chloride to remove protein from press cake at 5 掳C produced material with the highest protein content (83%), but extraction yield was 25%. When extractions were carried out at 77 掳C, oil typically began to be a major impurity in the protein. Using bomb calorimetry, the material remaining after extraction was found to have some value as a fuel source.