La France des pollens : l’apport décisif du Réseau national de surveillance aérobiologique
- 作者：G. Dutau
- 关键词：Tacrolimus ; LC– ; ESI-MS/MS ; Aqueous humor
- 刊名：Revue Francaise d'Allergologie et d'Immunologie Clinique
- 出版年：2008
- 期刊代码：239_03357457
- 类别：med
- 出版时间：June 2008
- 卷：48
- 期：4
- 页码：301-302
- 文件大小：84 K
摘要
A simple, sensitive and selective liquid chromatographic–tandem mass spectrometric (LC–MS/MS) method was developed for the determination of tacrolimus (FK506) in rabbit aqueous humor. After a simple protein-precipitation by methanol, the post-treatment samples were separated on a reversed-phase, Thermo-Hypersil-BDS-C18 column with a mobile phase of a mixture of 0.1%formic acid in water, methanol and acetonitrile (5:85:10, v/v/v). Tacrolimus and ritonavir (internal standard, IS) were all detected by the selected reaction-monitoring (SRM) mode. The method developed was validated in rabbit aqueous humor with a daily working range of 0.5–100 ng/ml with correlation coefficient, r > 0.99 and a sensitivity of 0.5 ng/ml as lower limit of quantification, respectively. This method was fully validated for the accuracy, precision, possible matrix effect and stability. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of tacrolimus in rabbit aqueous humor.