Prolongation of skin allograft survival in rats by the transplantation of microencapsulated xenogeneic neonatal porcine Sertoli cells

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• 作者：Giovanni Bistoni^a ; ^d ; Mario Calvitti^b ; Francesca Mancuso^b ; Iva Arato^b ; Giulia Falabella^b ; Rosa Cucchia^c ; Francesca Fallarino^b ; Alessio Becchetti^d ; Tiziano Baroni^b ; Stefania Mazzitelli^e ; Claudio Nastruzzi^e ; Maria Bodo^b ; Ennio Becchetti^b ; Don F. Cameron^f ; Giovanni Luca^b ; ¹ ; Riccardo Calafiore^g ; ¹ ; ^{islet@unipg.it}
• 关键词：Xenotrasplantation ; Skin allografts ; Microcapsules ; Sertoli cells
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：July, 2012
• 卷：33
• 期：21
• 页码：5333-5340
• 文件大小：2184 K

摘要

Skin rejection remains a major hurdle in skin reconstructive transplantation surgery. In fact, 85%of the grafted patients experience at least one episode of acute skin rejection in the first year. It has been observed that Sertoli cells (SC), when co-transplanted with allo- or xenogeneic cell/tissues, can induce graft acceptance in the absence of systemic immunosuppression. A method aimed at significantly prolonging skin allografts in rats transplanted with barium alginate-based microencapsulated xenogeneic porcine SC (SC-MCs) is described. Results demonstrated that intraperitoneal (IP) transplantation of SC-MCs with high cellular viability and function can significantly prolong allogeneic skin grafts when compared to transplantation controls receiving only empty alginate capsules (E-MCs). Lymphocytic infiltration at the skin graft site was not observed in 80%of the SC-MCs transplanted rats and these recipient animals showed a significant increased expression of T regulatory (Tregs) cells when compared to E-MCs transplantation controls. The findings of this report further substantiate the positive therapeutic effects of SC on transplantation technology mediated by Sertoli cell-induced alterations of the host's immune system and indicate new perspectives and new strategies for successful skin tissue allografts.