Escherichia coli lipopolysaccharides and Staphylococcus aureus enterotoxin B differentially modulate inflammatory microRNAs in bovine monocytes

详细信息	查看全文 | 推荐本文 |

• 作者：Francesca Dilda^a ; ¹ ; Gloria Gioia^a ; ¹ ; Laura Pisani^a ; Laura Restelli^a ; Cristina Lecchi^a ; Francesca Albonico^a ; Valerio Bronzo^a ; Michele Mortarino^a ; Fabrizio Ceciliani^a ; ^b ; ^{fabrizio.ceciliani@unimi.it}
• 关键词：miRNA ; Monocytes ; Lipopolysaccharide ; Staphylococcus aureus enterotoxin B ; Bovine
• 刊名：The Veterinary Journal
• 出版年：2012
• 期刊代码：338_10900233
• 类别：med
• 出版时间：June, 2012
• 卷：192
• 期：3
• 页码：514-516
• 文件大小：211 K

摘要

MicroRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including activation of cells of the immune system. This study investigated the effect of Escherichia coli lipopolysaccharide (LPS) and Staphylococcus aureus enterotoxin B (SEB) on the expression of five miRNAs involved in the inflammatory response, including miR-9, miR-125b, miR-155, miR-146a and miR-223, in bovine CD14⁺ cells (monocytes). Incubation of monocytes with SEB induced down-regulation of miR-155, miR-223 and miR-125b, but not the anti-inflammatory miRNA miR-146a. Conversely, incubation with LPS upregulated both miR-155 and miR-146a. In vitro incubation of isolated CD14⁺ bovine monocytes with LPS and SEB elicited different and opposite expression of miRNAs reportedly involved in inflammatory reactions.