Preparation and characterization of a novel 3D scaffold from poly(蓻-caprolactone)/biphasic calcium phosphate hybrid composite microspheres adhesion

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• 作者：Trinh-Quang Bao ; Rose Ann Franco ; Byong-Taek Lee ; ^{lbt@sch.ac.kr}
• 关键词：PCL/BCP-HCM 3D scaffold ; GF-SEDA ; In vitro study ; Composite ; Adhesion ; Porous
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 May, 2012
• 卷：64
• 期：Complete
• 页码：76-83
• 文件大小：1362 K

摘要

The use of scaffold-based strategies in tissue engineering applications requires that the micro-architecture of the scaffold satisfies key micro-structural and biological requirements. In this study, we examined the effects of experimental fabrication conditions on the synthesis of a scaffold formed by a poly(蓻-caprolactone)/biphasic calcium phosphate (PCL/BCP) hybrid composite microspheres adhesion. The goal of our study was to identify the optimal fabrication conditions. An optimized poly(蓻-caprolactone)/biphasic calcium phosphate hybrid composite microspheres (PCL/BCP-HCM) 3D scaffold was prepared using the gas foaming and spontaneous emulsion droplets adherence (GF-SEDA) technique. Optical microscopy, 渭CT, SEM, mercury porosimeter, EDS, XRD, a universal testing machine, and MTT assays were used to characterize the assembled scaffolds. When the GF-SEDA technique was used under optimal conditions, the resulting novel PCL/BCP-HCM 3D scaffold had a micro-porous homogenous structure composed of PCL and BCP with a large distribution of pore sizes (ranging from 0.01 to 1000 渭m) and highly interconnected micro-pores. In addition, the total porosity of the scaffold was 74%with a compressive strength of 0.82 MPa. Furthermore, an in vitro study of cell-scaffold interaction demonstrated that cell adhesion, growth, and proliferation were significant over the course of 5 days in culture.