Dual effects of TNFα on nerve fiber formation from ventral mesencephalic organotypic tissue cultures
- 作者：Franziska Marschinke ; Ingrid Strö ; mberg
- 关键词：Ventral mesencephalon ; TNF
//www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0"> ; Dopamine ; Nerve fiber formation
- 刊名：Brain Research
- 出版年：2008
- 期刊代码：8_00068993
- 类别：neu
- 出版时间：18 June 2008
- 卷：1215
- 期：Complete
- 页码：30-39
- 文件大小：1453 K
摘要
Tumor necrosis factor alpha (TNF
k small letter alpha" title="greek small letter alpha" border="0">) is toxic to dopamine neurons and increased levels of TNFk small letter alpha" title="greek small letter alpha" border="0"> are observed in Parkinson's disease. Dopamine nerve fiber outgrowth in organotypic cultures of fetal ventral mesencephalon occurs in two waves. The early appearing nerve fibers are formed in the absence of astroglia, while migrating astrocytes guide the late appearing dopamine nerve fibers. TNFk small letter alpha" title="greek small letter alpha" border="0"> (40 ng/ml) was added to the medium of organotypic ventral mesencephalic tissue cultures between days 4–7 and 11–14. The cultures were evaluated at days 7 or 19 to study the effects of TNFk small letter alpha" title="greek small letter alpha" border="0"> on both types of nerve fiber formation. Tyrosine hydroxylase (TH)-immunohistochemistry demonstrated that the number of cultures showing non-glial-guided TH-positive outgrowth was reduced compared to controls, when TNFk small letter alpha" title="greek small letter alpha" border="0"> was added at day 4. By contrast, the glial-guided TH-positive nerve fiber outgrowth and the astrocytic migration reached significantly longer distances by early TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment. Ki67-immunohistochemistry revealed that TNFk small letter alpha" title="greek small letter alpha" border="0"> did not affect proliferation of astrocytes. Treatment with TNFk small letter alpha" title="greek small letter alpha" border="0"> and antibodies against TNFk small letter alpha" title="greek small letter alpha" border="0"> receptor 1 between days 4 and 7 revealed that the non-glial-guided TH-positive outgrowth reappeared. TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment between days 11 and 14 triggered neither the TH-positive glial-guided outgrowth, nor promoted the astrocytic migration to reach longer distances. The number of microglia was significantly increased after the late but not early TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment. In conclusion, TNFk small letter alpha" title="greek small letter alpha" border="0"> is toxic for the non-glial dopaminergic nerve fiber outgrowth but stimulates the glial-guided outgrowth and the migration of astrocytes at an early time point. TNFk small letter alpha" title="greek small letter alpha" border="0"> increased the number of microglia in VM tissue cultures after late but not after early treatment.
k small letter alpha" title="greek small letter alpha" border="0">) is toxic to dopamine neurons and increased levels of TNFk small letter alpha" title="greek small letter alpha" border="0"> are observed in Parkinson's disease. Dopamine nerve fiber outgrowth in organotypic cultures of fetal ventral mesencephalon occurs in two waves. The early appearing nerve fibers are formed in the absence of astroglia, while migrating astrocytes guide the late appearing dopamine nerve fibers. TNFk small letter alpha" title="greek small letter alpha" border="0"> (40 ng/ml) was added to the medium of organotypic ventral mesencephalic tissue cultures between days 4–7 and 11–14. The cultures were evaluated at days 7 or 19 to study the effects of TNFk small letter alpha" title="greek small letter alpha" border="0"> on both types of nerve fiber formation. Tyrosine hydroxylase (TH)-immunohistochemistry demonstrated that the number of cultures showing non-glial-guided TH-positive outgrowth was reduced compared to controls, when TNFk small letter alpha" title="greek small letter alpha" border="0"> was added at day 4. By contrast, the glial-guided TH-positive nerve fiber outgrowth and the astrocytic migration reached significantly longer distances by early TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment. Ki67-immunohistochemistry revealed that TNFk small letter alpha" title="greek small letter alpha" border="0"> did not affect proliferation of astrocytes. Treatment with TNFk small letter alpha" title="greek small letter alpha" border="0"> and antibodies against TNFk small letter alpha" title="greek small letter alpha" border="0"> receptor 1 between days 4 and 7 revealed that the non-glial-guided TH-positive outgrowth reappeared. TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment between days 11 and 14 triggered neither the TH-positive glial-guided outgrowth, nor promoted the astrocytic migration to reach longer distances. The number of microglia was significantly increased after the late but not early TNFk small letter alpha" title="greek small letter alpha" border="0"> treatment. In conclusion, TNFk small letter alpha" title="greek small letter alpha" border="0"> is toxic for the non-glial dopaminergic nerve fiber outgrowth but stimulates the glial-guided outgrowth and the migration of astrocytes at an early time point. TNFk small letter alpha" title="greek small letter alpha" border="0"> increased the number of microglia in VM tissue cultures after late but not after early treatment.