Morphometric investigations of the mitochondrial damage in ceroid lipopigment accumulation due to vitamin E deficiency
- 作者：Bertoni-Freddari ; Carlo ; Fattoretti ; Patrizia ; Casoli ; Tiziana ; Di Stefano ; Giuseppina ; Solazzi ; Moreno ; et. al.
- 关键词：Numeral density (Nv) ; Volume density (Vv) ; Average size of synaptic mitochondria ; Vitamin E deficient rats ; Computer-assisted morphometry ; Cerebellar granular layer ; Purkinje cells ; Accumulation of ceroid pigment
- 刊名：Archives of Gerontology and Geriatrics
- 出版年：2002
- 期刊代码：23_01674943
- 类别：med
- 出版时间：May - June, 2002
- 卷：34
- 期：3
- 页码：269-274
- 文件大小：100 K
摘要
Numeric (Nv) and volume (Vv) densities, as well as the average size (skeleton: Sk) of synaptic mitochondria from adult, normally fed and adult, vitamin E deficient animals (11 months of age) were semiautomatically measured by computer-assisted morphometry in the cerebellar granular layer. Nv, Vv and the average mitochondrial volume (V) were measured on perikaryal Purkinje cell organelles preferentially stained for succinic dehydrogenase (SDH) activity. Adult vitamin E deficient animals showed a significant decrease of Nv, a significant increase of Sk and an unchanged value of Vv. While in adult normally fed animals the mitochondria of increased size (Sk>5 μm) were 5.3%, in the adult vitamin E deficient rats this fraction accounted for 25.5%. In Purkinje cell perikarya, vitamin E deficiency resulted in a significant decrease of Vv, Nv and V, as well as a steeper reduction of the percentage of SDH-positive mitochondria of larger size. Taken together, these findings document that vitamin E deficiency is responsible of mitochondrial morphometric alterations in adult rats. Structurally deteriorated mitochondria are reported to play a role in producing increased amounts of free radicals, which can facilitate the accumulation of ceroid pigment.