Influence of extender, freezing rate, and thawing rate on post-thaw motility, viability and morphology of coyote (Canis latrans) spermatozoa
- 作者：Larry J. Minter and Thomas J. DeLiberto
- 关键词：Coyote ; Semen cryopreservation ; Extender ; Freezing rates ; Thawing rates
- 刊名：Theriogenology
- 出版年：2005
- 期刊代码：98_0093691x
- 类别：abs
- 出版时间：December 2005
- 卷：64
- 期：9
- 页码：1898-1912
- 文件大小：161 K
摘要
The objective of this study was to examine the post-thaw effects of three cryoprotective extenders (Tris–fructose–citric acid extender, Tris–glucose–citric acid extender, and lactose extender), three linear freezing rates (−1, −6, and −20 °C/min), and three thawing rates (37 °C water bath for 120 s, 60 °C water bath for 30 s, and 70 °C water bath for 8 s) on coyote spermatozoa. After thawing, the findings supported that cryopreservation of coyote (Canis latrans) spermatozoa frozen at a moderate freezing rate (−6 °C/min), in either a Tris–fructose or Tris–glucose extender, and thawed at a slow rate (37 °C water bath for 120 s) or moderate rate (60 °C water bath for 30 s), resulted in a more vigorous post-thaw motility (range, 57.5–44.0%) and viability (range, 64–49.6%) with the least amount of morphological and acrosomal abnormalities.