The advantages of using a combination of LDL and glutamine in comparison with TRIS egg yolk and Equex庐 STAMP extenders in the cryopreservation of canine semen

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• 作者：Djemil Bencharif^a ; ^{djemil.bencharif@oniris-nantes.fr} ; Lamia Amirat-Briand^a ; Annabelle Garand^a ; Marc Anton^b ; Eric Schmitt^c ; Serge Desherces^c ; Guy Delhomme^c ; Marie-Laure Langlois^d ; Paul Barriè ; re^d ; Sandrine Destrumelle^a ; Oscar Vera-Munoz^a ; Daniel Tainturier^a
• 关键词：Canine semen ; Cryopreservation ; LDL ; Glutamine ; TRIS egg yolk ; Equex STAMP ; Vital stains
• 刊名：Research in Veterinary Science
• 出版年：2012
• 期刊代码：272_00345288
• 类别：med
• 出版时间：August, 2012
• 卷：93
• 期：1
• 页码：440-447
• 文件大小：506 K

摘要

Twenty semen samples taken from 5 dogs were frozen in liquid nitrogen at 鈭?96 掳C in four different extenders: one control extender based on 20%egg yolk, 6%LDL alone (low density lipoproteins: the active cryoprotective principle in chicken egg yolk), 6%LDL combined with 20 mmol glutamine, and Equex庐 (a reference extender that we wish to compare with the LDL-glutamine combination). After thawing, spermatozoal motility was evaluated using a HAMILTON THORNE CERROS 12 image analyzer; the percentage of motile spermatozoa was 27.7%in the egg yolk extender (p < 0.05), 49.9%with 6%LDL alone (p > 0.05), 54.7%in the 6%LDL + 20 mmol glutamine extender, and 47.9%with Equex庐 (p > 0.05). The motility parameters (VAP, VCL, VSL and ALH) were also superior in the 6%LDL + 20 mmol glutamine extender in comparison with the other extenders.

Finally, the spermatozoa were generally better protected during freezing with the 6%LDL + 20 mmol glutamine association than with the egg yolk, 6%LDL, or Equex extenders in terms of the flagellar plasma membrane (HOS test), DNA (Acridine orange test), and acrosome integrity (Spermac庐 test: no significant difference). The Equex庐 extender obtained the best results for the acrosome, followed by 6%LDL + 20 mmol glutamine (FITC-PSA test: p < 0.05 between each extender).