Sperm concentration at freezing affects post-thaw quality and fertility of ram semen

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• 作者：M. Alvarez^a ; ^b ; J. Tamayo-Canul^a ; ^b ; E. Anel^a ; ^b ; J.C. Boixo^c ; M. Mata-Campuzano^a ; ^b ; F. Martinez-Pastor^a ; ^d ; ^{felipe.martinez@unileon.es} ; L. Anel^a ; ^b ; P. de Paz^a ; ^d
• 关键词：Ram ; Sperm cryopreservation ; Sperm concentration ; Sperm quality ; Fertility
• 刊名：Theriogenology
• 出版年：2012
• 期刊代码：98_0093691x
• 类别：abs
• 出版时间：1 April, 2012
• 卷：77
• 期：6
• 页码：1111-1118
• 文件大小：718 K

摘要

We have investigated the effect of sperm concentration in the freezing doses 200, 400, 800, and 1600 脳 10⁶ mL^鈭? on the post-thaw quality and fertility of ram semen. Semen was collected from seven adult Churra rams by artificial vagina during the breeding season. The semen was diluted in an extender (TES-Tris-fructose, 20%egg yolk, and 4%glycerol), to a final concentration of 200, 400, 800, or 1600 脳 10⁶ mL^鈭? and frozen. Doses were analyzed post-thawing for motility (computer-assisted sperm analysis system [CASA]), viability, and acrosomal status (fluorescence probes propidium iodide [PI]/peanut agglutinin conjugated with fluorescein thiocyanate (PNA-FITC), SYBR-14/PI [Invitrogen; Barcelona, Spain] and YO-PRO-1/PI [Invitrogen; Barcelona, Spain]). Total motility and velocity were lower for 1600 脳 10⁶ mL^鈭? doses, while progressive motility and viability were lower both for 800 and 1600 脳 10⁶ mL^鈭?. The proportion of viable spermatozoa showing increased membrane permeability (YO-PRO-1+) rose in 800 and 1200 脳 10⁶ mL^鈭?. Intrauterine inseminations were performed with the 200, 400, and 800 脳 10⁶ mL^鈭? doses at a fixed sperm number (25 脳 10⁶ per uterine horn) in synchronized ewes. Fertility (lambing rate) was similar for semen frozen at 200 (57.5%) or 400 脳 10⁶ mL^鈭? (54.4%), whereas it was significantly lower for 800 脳 10⁶ mL^鈭? (45.5%). In conclusion, increasing sperm concentration in cryopreserved semen, at least at 800 脳 10⁶ mL^鈭? and more, adversely affects the postthawing quality and fertility of ram semen.