Physical and functional interactions between STAT3 and Kaposi’s sarcoma-associated herpesvirus-encoded LANA

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• 作者：Ryuta Muromoto ; Kanako Okabe ; Masahiro Fujimuro ; Kenji Sugiyama ; Hideyoshi Yokosawa ; Tsukasa Seya and Tadashi Matsuda
• 关键词：Signal transducer and activator of transcription 3 ; Kaposi&#x2019 ; s sarcoma-associated herpesvirus ; Latency-associated nuclear antigen ; Transcription
• 刊名：FEBS Letters
• 出版年：2006
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：9 January 2006
• 卷：580
• 期：1
• 页码：93-98
• 文件大小：308 K

摘要

The Kaposi’s sarcoma-associated herpesvirus (KSHV)-encoded latency-associated nuclear antigen (LANA) is known to modulate viral and cellular gene expression. We show that LANA directly associates with an interleukin-6 signal transducer, signal transducer and activator of transcription 3 (STAT3) and that LANA enhances the transcriptional activity of STAT3. Coimmunoprecipitation studies documented a physical interaction between LANA and STAT3 in transiently transfected 293T cells as well as the KSHV-infected primary effusion lymphoma (PEL) cell line. Furthermore, small-interfering RNA-mediated reduction of LANA expression decreased the STAT3-dependent transcription in KSHV-positive PEL cells, whereas overexpression of LANA enhanced STAT3 activity in KSHV-negative B lymphoma cells. These data demonstrate that LANA is a transcriptional co-activator of STAT3, and may have implications for the pathogenesis of KSHV-associated diseases.