PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin
- 作者：Spano ; Furio ; Putignani ; Lorenza ; McLauchlin ; James ; Casemore ; David P. ; Crisanti ; Andrea
- 关键词：Cryptosporidium wrairi ; Cryptosporidium parvum ; Cryptosporidium oocyst wall protein gene ; Genetic marker ; Polymerase chain reaction
- 刊名：FEMS Microbiology Letters
- 出版年：1997
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：May 15, 1997
- 卷：150
- 期：2
- 页码：209-217
- 文件大小：748 K
摘要
Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptosporidium oocyst wall protein (COWP) of C. wrairi, which showed 98%identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.