Clotting factor activity in thawed Octaplas庐 LG during storage at 2-6 掳C for 6 days from a quality assurance point of view

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• 作者：Mareike Kristina Keller^a ; ^{mareike.keller@charite.de} ; Martin Krebs^a ; Claudia Spies^a ; Klaus-Dieter Wernecke^c ; ^{klaus.wernecke@gmx.de} ; Andrea Heger^b ; Christian von Heymann^a ; ^{christian.von_heymann@charite.de}
• 关键词：S/D plasma ; Clotting factors ; Plasma storage
• 刊名：Transfusion and Apheresis Science
• 出版年：2012
• 期刊代码：248_14730502
• 类别：med
• 出版时间：April, 2012
• 卷：46
• 期：2
• 页码：129-136
• 文件大小：331 K

摘要

Introduction

Octaplas庐 LG is a second-generation solvent/detergent-treated plasma that offers an additional safety benefit by prion elimination. The stability of clotting factors of the new S/D plasma after thawing has not been investigated yet. This study intended to measure the time course of fibrinogen, FII, FV, FVII, FVIII, FIX, PC, fPS and PI through storage at 2-6 掳C over 6 days.

Materials and methods

We investigated 20 plasma bags (five bags per blood group) and measured fibrinogen, FII, FV, FVII, FVIII, FIX, PC, fPS and PI immediately after thawing and after 2, 4, 6, 24, 48, 72, 96, 120 and 144 h storage at 2-6 掳C. Five separate plasma bags were thawed and stored at 2-6 掳C for microbiological assessment. After 6 days samples were drawn for blood cultures that were incubated for six more days.

Results

After 6 days FII, FIX and PC showed no significant changes. FV (鈭?6%, p < 0.001), FVII (鈭?9%, p < 0.001), FVIII (鈭?9%, p < 0.001), FXI (鈭?3%, p < 0.0001) and fPS (鈭?%, p < 0.0007) decreased significantly. PI levels were stable at 56%. The microbiological investigation showed no bacterial contamination.

Conclusions

In Octaplas庐 LG plasma clotting factors decreased slightly through storage of 6 days. PI levels were remarkably higher and stable over time in the new Octaplas庐 LG. Stability of stored Octaplas庐 LG was limited by the decrease of FVIII to 53%, which may warrant storage up to 24 h from a quality assurance point of view. This could result in reduced plasma wastage and costs for healthcare givers.