Stable expression of green fluorescent protein mediated by GCV in Giardia canis
- 作者：Chengwu Liu ; Jianhua Li ; Xichen Zhang ; Quan Liu ; Hui Liu ; Pengtao Gong ; Guocai Zhang ; Longquan Yao ; Xinxin Zhang
- 关键词：Giardia canis ; GCV ; Green fluorescent protein ; Neomycin phosphotransferase
- 刊名：Parasitology International
- 出版年：2008
- 期刊代码：125_13835769
- 类别：bio
- 出版时间：September 2008
- 卷：57
- 期：3
- 页码：320-324
- 文件大小：296 K
摘要
Giardia canis can be infected with a double-stranded RNA virus, that is giardiavirus (G. canis virus, GCV). In this study, green fluorescent protein (GFP) was stably expressed in G. canis mediated by GCV. The plasmid pNEO/GDH/MCS/GFP, containing the neomycin phosphotransferase (NEO) encoding region flanked by the 636 nt of 5′-terminus and the 2174 nt of 3′-terminus from GCV positive strand RNA, was constructed by inserting GFP gene into downstream from the NEO gene and glutamate dehydrogenase (GDH) 5′-terminus uncoding regions on a single plasmid, and its in vitro transcript was introduced into GCV-infected G. canis by electroporation. The transfectants expressed GFP persistently under G418 selection. This stable transfection system should provide a valuable tool for genetic study of G. canis.