A series of muscarinic agonists, straight chained, branched, cyclic alkyl and aromatic derivatives of the oxime
1 (demox) was designed with the aim of investigating their activity on muscarinic receptor subtypes. Effects on M
1 receptor were assessed functionally by a microphysiometer apparatus, while M
2, M
3, and M
4 receptor potency and affinity were studied on isolated preparations of guinea pig heart, ileum, and lung, respectively. The results suggest that the substitution of a hydrogen with a long side-chain or bulky group generally induces a decrease in potency at M
1 and M
3 subtypes, while a general increase in this parameter is obtained at M
2 subtype. Among the agonists
2–
18, compound
4 behaves as a full agonist with a preference for M
3 subtype. Moreover, compound
12 is inactive at M
1 and M
4 receptors while it displays a full agonist activity at M
2 and M
3 subtypes. Since demox displays a variable response on cardiac M
2 receptors regulating heart force, an in-depth inquiry of the functional behaviour of this compound was carried out at M
2 receptors. In presence of 10
−11 and 10
−10 M demox, the binding of [
3H]-NMS was increased by
30%as a consequence of an increase of the association of [
3H]-NMS to membranes; this effect was not observed in presence of a higher concentration of [
3H]-NMS. Higher concentrations of demox decreased the binding of [
3H]-NMS to heart atrial membranes but significantly retarded the dissociation of this radioligand. Our results suggest that demox may interact with orthosteric and allosteric sites of atrial M
2 muscarinic receptor.