Counter-current chromatography separation scaled up from an analytical column to a production column
- 作者：Philip Wood ; Svetlana Ignatova ; Lee Janaway ; David Keay ; David Hawes ; Ian Garrard ; Ian A. Sutherl
- 关键词：Counter-current chromatography ; Scale up ; Mass transfer ; High-performance liquid chromatography ; High-performance counter-current chromatography
- 刊名：Journal of Chromatography A
- 出版年：2007
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：1 June 2007
- 卷：1151
- 期：1-2
- 页码：25-30
- 文件大小：216 K
摘要
An analytical separation was performed on an analytical J-type counter-current chromatography (CCC) instrument using a 5.4 ml column, with a 1 ml/min mobile phase flow rate. This separation had a resolution of 0.69 and was achieved in 10 min. The same separation was performed using two 2300 ml columns connected in series at a flow rate of 850 ml/min using a production scale J-type centrifuge. This production scale separation was also obtained in 10 min with a resolution of 0.71. This represents an 850 times increase in productivity. This paper presents these separations and the underlying scale up theory.