Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells

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• 作者：Yuling Yin^a ; ¹ ; Gina Borges^b ; Masaaki Sakuta^a ; Alan Crozier^b ; Hiroshi Ashihara^a ; ^{ashihara.hiroshi@ocha.ac.jp} ; ^{hiroshi.ashihara@gmail.com}
• 关键词：Vitis sp. ; Vitaceae ; Grape ; Biosynthesis ; Anthocyanin ; Cyanidin-3-O-glycosides ; Peonidin-3-O-glycosides ; Phenylalanine ; Gene expression
• 刊名：Plant Physiology and Biochemistry
• 出版年：2012
• 期刊代码：87_09819428
• 类别：abs
• 出版时间：June, 2012
• 卷：55
• 期：Complete
• 页码：77-84
• 文件大小：404 K

摘要

The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O状-p-coumaroyl)-glucoside and peonidin-3-O-(6-O状-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (鈭扨i) medium but accumulation was much more substantial cells in聽鈭扨i cultures. Highest incorporation of [ring-¹⁴C]phenylalanine into anthocyanins was found at days 3-14 in the cells in聽鈭扨i medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the聽+Pi and聽鈭扨i medium. The transcript levels of UFGT and VvmybA1 in聽鈭扨i cells were higher than in聽+Pi cells 24聽h after transfer coinciding with the start of anthocyanin accumulation in聽鈭扨i cells.