In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines
- 作者：S.H. Doak ; s.h.doak@swansea.ac.uk ; B. Manshian ; G.J.S. Jenkins ; N. Singh
- 关键词：Nanoparticles ; Nanotubes ; Nanomaterials ; Genotoxicity ; Micronucleus assay ; Ames test
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2012
- 期刊代码：31_13835718
- 类别：pha
- 出版时间：14 June, 2012
- 卷：745
- 期：1-2
- 页码：104-111
- 文件大小：325 K
摘要
There is a pressing requirement to define a hazard identification and risk management strategy for nanomaterials due to the rapid growth in the nanotechnology industry and their promise of life-style revolutions through the development of wide-ranging nano-containing consumer products. Consequently, a battery of well defined and appropriate in vitro assays to assess a number of genotoxicity endpoints is required to minimise extensive and costly in vivo testing. However, the validity of the established protocols in current OECD recognised genotoxicity assays for nanomaterials is currently being questioned. In this report, we therefore consider the in vitro OECD genotoxicity test battery including the Ames, micronucleus and HPRT forward mutation assays, and their potential role in the safety assessment of nanomaterial induced DNA damage in vitro.