Selective high-performance liquid chromatographic assay for itraconazole and hydroxyitraconazole in plasma from human immunodeficiency virus-infected patients
- 作者：Koks ; Cees H.W. ; Sparidans ; Rolf W. ; Lucassen ; Georgette ; Crommentuyn ; Kristel M.L. ; Beijnen ; Jos H.
- 关键词：Itraconazole ; Hydroxyitraconazole
- 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2002
- 期刊代码：124_15700232
- 类别：ch
- 出版时间：February 5, 2002
- 卷：767
- 期：1
- 页码：103-110
- 文件大小：117 K
摘要
A sensitive and selective reversed-phase liquid chromatographic assay for itraconazole and hydroxyitraconazole in human plasma has been developed and validated. Itraconazole and hydroxyitraconazole were extracted from the matrix using solid-phase extraction on a strong cation-exchange sorbent. All compounds were detected using fluorescence at 265 and 363 nm for excitation and emission, respectively. The assay has been validated over the range 10–1000 ng/ml for both compounds, 10 ng/ml being the lower limit of quantification. Accuracies ranged from 104 to 113%for itraconazole and from 91 to 103%for hydroxyitraconazole. The intra-assay precisions were all below 9%for itraconazole and below 8%for hydroxyitraconazole. The selectivity has been evaluated with respect to all registered anti-human immunodeficiency virus (HIV) drugs and other potential co-medications and a few of their metabolites, commonly used by HIV-infected individuals. Both itraconazole and hydroxyitraconazole were stable under relevant conditions for HIV-inactivation and storage of samples. The applicability of the assay was demonstrated for samples collected from a treated HIV-infected patient.