Identification of the bacterial etiology of culture-negative endocarditis by amplification and sequencing of a small ribosomal RNA gene

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• 作者：Khulordava ; Irakli ; Miller ; Geraldine ; Haas ; David ; Li ; Haijing ; McKinsey ; Joel ; Vanderende ; Daniel ; et. al.
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2003
• 期刊代码：79_07328893
• 类别：med
• 出版时间：May, 2003
• 卷：46
• 期：1
• 页码：9-11
• 文件大小：82.8 K

摘要

We report two cases of culture-negative bacterial endocarditis in which the organisms were identified by amplification and sequencing of the bacterial 16S rRNA gene. These results support an important role for polymerase chain reaction followed by direct sequencing to determine the etiology of culture-negative bacterial endocarditis and to guide appropriate antimicrobial therapy.