Evaluation of antinociceptive effects of Crassocephalum bauchiense Hutch (Asteraceae) leaf extract in rodents

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• 作者：Germain Sotoing Ta&#239 ; we^a ; ^b ; ^c ; ^{taiwe_sotoing@yahoo.fr} ; Elisabeth Ngo Bum^d ; Emmanuel Talla^e ; Thé ; ophile Dimo^b ; Neteydji Sidiki^d ; Amadou Dawe^f ; Richard Marcel Nguimbou^g ; Paul Dé ; siré ; Djomeni Dzeufiet^b ; Michel De Waard^c ; ^h
• 关键词：AE ; aqueous extract ; AF ; alkaloid fraction ; Fr ; fraction ; ID50 ; dose of extract necessary to reduce the response by 50%relative to the control value ; LD50 ; median lethal dose ; NIH ; National Institutes of Health ; S.E.M. ; standard error of the means ; v ; vo
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：7 May, 2012
• 卷：141
• 期：1
• 页码：234-241
• 文件大小：728 K

摘要

Ethnopharmacological relevance

The leaves of Crassocephalum bauchiense have long been used in traditional Cameroonian medicine for the treatment of epilepsy, pain, inflammatory disorders, arthritis and intestinal pain.

Aim of the study

In this study, we attempted to identify the possible antinociceptive action of the aqueous extract and the alkaloid fraction prepared from the leaves of Crassocephalum baucheiense.

Materials and methods

Using acetic acid induced abdominal constrictions, formalin-, capsaisin- and glutamate-induced nociception, and hot plate assay procedures, the antinociceptive effects of the aqueous extract and the alkaloid fraction was assessed after oral administration in mice. Morphine sulfate was used as reference analgesic agent. Mice were submitted to the rota-rod task and open-field test in order to assess any non-specific muscle-relaxant or sedative effects of the extracts of Crassocephalum bauchiense. Male and female Swiss mice were used to assess acute toxicity of these extracts.

Results

The aqueous extract and the alkaloid fraction of Crassocephalum bauchiense produced a significant antinociceptive effects in the acetic acid, formalin, glutamate, capsaicin and hot plate tests. These antinociceptive effects of Crassocephalum bauchiense were significantly attenuated by pretreatment with naloxone. The extracts of Crassocephalum bauchiense did not alter the locomotion of animals in the open-field or rotarod tests, which suggest a lack of a central depressant effect. The animals did not exhibit any acute toxicity to the aqueous extract and the alkaloid fraction, so it was not possible to calculate the LD₅₀.

Conclusion

The results confirm the popular use of Crassocephalum bauchiense as an antinociceptive, and contribute to the pharmacological knowledge of this species because it was shown that the aqueous extract and the alkaloid fraction of Crassocephalum bauchiense produced dose related antinociception in models of chemical and thermal nociception through mechanisms that involve an interaction with opioidergic pathway.