Hydrocortisone does not affect major platelet receptors in inflammation in vitro

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• 作者：Tobias Schuerholz ; Oliver Keil ; Tobias Wagner ; Stefan Klinzing ; Robert S&#xfc ; mpelmann ; Volker Oberle ; Gernot Marx
• 关键词：Sepsis ; drug effects ; In vitro ; Platelet membrane glycoproteins
• 刊名：Steroids
• 出版年：2007
• 期刊代码：142_0039128x
• 类别：bio
• 出版时间：June 2007
• 卷：72
• 期：6-7
• 页码：609-613
• 文件大小：186 K

摘要

Hypothesis

Platelet function is an important factor for the fate of intensive care patients. Several factors may influence this function. Recently, it was demonstrated that hydrocortisone has immunologic effects in septic shock and therefore may affect cell adhesion molecules. The aim of the present study was to examine effects of hydrocortisone on platelet receptor expression in healthy individuals and septic patients in vitro.

Methods

Citrated blood samples were drawn from 10 healthy volunteers and 10 septic patients. Samples were adjusted with hydrocortisone to final concentrations of 4.5 μg mL⁻¹, 9.0 μg mL⁻¹ (sepsis-equivalent bolus) and 90 μg mL⁻¹, respectively. A control group received no additional hydrocortisone. Expression of CD62P, CD41, PAC-1 and CD42b on the surface of resting or agonist-stimulated platelets was determined by whole blood flow cytometry using fluorescence-labeled monoclonal antibodies.

Results

Hydrocortisone had no significant influence on the expression of CD62P, CD41 and PAC-1. After administration of 90 μg mL⁻¹ hydrocortisone the expression of CD42b was decreased compared to controls after activation. Differences between volunteers and sepsis patients were found for all receptors after activation.

Conclusions

Hydrocortisone mediates immunmodulating effects in therapy of patients suffering of septic shock without involvement of specific platelet receptors in vitro.