Distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) (Castelnau, 1872)

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• 作者：Catarina Norte dos Santos^a ; Melanie Leef^a ; ^{Melanie.Leef@utas.edu.au} ; Brian Jones^b ; Nathan Bott^c ; Daniele Giblot-Ducray^c ; Barbara Nowak^a
• 关键词：Southern bluefin tuna ; Cardicola forsteri ; Sanguinicolidae ; Gill histpathology
• 刊名：Aquaculture
• 出版年：2012
• 期刊代码：16_00448486
• 类别：abs
• 出版时间：21 May, 2012
• 卷：344-349
• 期：Complete
• 页码：54-57
• 文件大小：361 K

摘要

The distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) was analysed. Eggs were confirmed to be C. forsteri using laser-capture microdissection and quantitative polymerase chain reaction (qPCR) analyses. Prevalence of infection was significantly higher in the second gill arch (蠂² = 6.49, P < 0.05) than in the fourth gill arch, and significantly lower in the basal region of the second gill arch (蠂² = 6.29, d.f. = 2, P < 0.05). The intensity of infection was not significantly different between the gill arches (F = 0.03, d.f. = 1, P > 0.05). Similarly, there was no significant difference in the intensity of C. forsteri eggs between the gill arches (F = 3.43, d.f. = 2, P > 0.05), or at different depth of sectioning (F = 0.08, d.f. = 1.12, P > 0.05). Results suggest that the presence of C. forsteri eggs in the gills of tuna is more likely to be detected by sampling the second gill arch. Furthermore, targeting the middle region and increasing the sectioning depth may reduce the proportion of false negatives.