Detection of Toscana virus central nervous system infections in Sardinia Island, Italy

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• 作者：Giulietta Venturi ; Giordano Madeddu ; Giovanni Rezza ; Massimo Ciccozzi ; Maria Laura Pettinato ; Marco Cilliano ; Cristiano Fiorentini ; Maria Stella Mura ; Maria Grazia Ciufolini
• 关键词：Toscana virus ; Central nervous system disease ; Diagnosis ; Sardinia Island
• 刊名：Journal of Clinical Virology
• 出版年：2007
• 期刊代码：150_13866532
• 类别：med
• 出版时间：September 2007
• 卷：40
• 期：1
• 页码：90-91
• 文件大小：117 K

摘要

The magnitude of specific CD8+ T cell reactivity responsible for vaccine-induced protection against smallpox infection has not yet been fully elucidated. Among other techniques, RT-PCR for the monitoring of cytokine release in effector T cells against tumor and viral antigens has demonstrated a novel promising method.

Objective

To determine the functional status of antigen specific CD8+ T cells in healthy participants before and 4 weeks after prophylactic vaccination (Lister strain) against smallpox using quantitative real-time PCR (qRT-PCR).

Study design

Changes of interferon-γ (IFNγ) mRNA expression levels on short term ex vivo peptide antigen stimulation were measured. The corresponding specific CD8+ T cell reactivity was then displayed as CD8-normalized IFN-γ levels (IFN-γ/CD8 ratio).

Results

We found a 5–9 fold increase of CD8+ T cell reactivity in three out of four vaccinated individuals. The kinetics and strength determined in responders reveal a virus specific T cell effector repertoire pre-vaccination and a corresponding functional state after immunization comparable also to data obtained from tetramer- and ELISPOT analysis.

Conclusions

Apart from protective vaccinia-specific neutralizing antibodies, the presence of antigen-specific CD8+ T-cells has been demonstrated after vaccinia vaccination. In concordance with others, results from this PCR-based study indicate that this smallpox vaccine induces strong vaccinia virus-specific CD8+ and IFN-γ producing T cell responses.