Suppression of error prone pathway is responsible for antimutagenic activity of honey

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• 作者：Sudhanshu Saxena^a ; Satyendra Gautam^a ; Girish Maru^b ; Dhananjay Kawle^b ; Arun Sharma^a ; ^{ksarun@barc.gov.in}
• 关键词：Pollen analysis ; Antimutagenicity ; Ames ; rpoB/RifR ; SOS response ; SIVET assay
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：March-April, 2012
• 卷：50
• 期：3-4
• 页码：625-633
• 文件大小：617 K

摘要

Honey, both unifloral (Syzygium cumini) and bifloral, demonstrated strong antimutagenicity against physical (UV, 纬) and chemical (ethylmethane sulfonate) mutagens as ascertained by rpoB/Rif^R and Ames tests. The effect of honey was evaluated in radiation (UV or 纬) exposed Escherichia coli cells for SOS response, a well known error prone repair pathway known to significantly contribute to mutagenicity by quantifying LexA repressor level, measuring cell filamentation frequency, and prophage induction by SIVET (Selectable - In-Vivo Expression Technology) assay. LexA was almost completely degraded, phenotypically long filamentous cells (鈭?0 渭m) were formed, and SIVET induction frequency was increased in radiation exposed E. coli cultures, however, these changes were significantly inhibited in presence of honey confirming its strong antimutagenic nature. Further, rpoB/Rif^R mutation frequency upon UV exposure in E. coli recA^{鈭?/sup> cells was found to be negligible, whereas, E. coli umuC鈭?/sup> and umuD鈭?/sup> knockouts showed comparatively higher mutation frequency. Honey did not show any effect on mutagenesis in these knockouts, indicating the SOS dependence of the observed mutagenesis. Honey was also found to suppress EMS induced mutagenesis but through SOS independent mechanism. Phenolics present in honey were found to be one of the important factors contributing to the antimutagenicity of honey.}