Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp
- 作者：Picardeau ; Mathieu ; Bauby ; Hé ; lè ; ne ; Saint Girons ; Isabelle
- 关键词：Leptospira ; Methionine ; Transsulfuration ; Direct sulfhydrylation
- 刊名：FEMS Microbiology Letters
- 出版年：2003
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：August 29, 2003
- 卷：225
- 期：2
- 页码：257-262
- 文件大小：321 K
摘要
There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes a protein exhibiting similarities with homologs in many organisms belonging to the α-, β-, and γ-subdivisions of proteobacteria. The metW, metX and metY genes of L. meyeri were disrupted by a resistance cassette by homologous recombination. While the L. meyeri metX mutant shows methionine auxotrophy, the metY mutant (as well as the metW and metYmetW mutants) conserves methionine prototrophy, suggesting the presence of additional route(s) which may bypass the direct sulfhydrylation pathway. In addition, a L. interrogans gene, called metZ, was found to complement an Escherichia coli metB mutant, further suggesting that the transsulfuration pathway is also present in Leptospira spp.