Localization of the Leptospira interrogans metF gene on the CII secondary chromosome

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• 作者：Bourhy ; Pascale ; Saint Girons ; Isabelle
• 关键词：Leptospira interrogans ; metF ; Methylene tetrahydrofolate reductase ; Thermolabile enzyme ; Secondary chromosome
• 刊名：FEMS Microbiology Letters
• 出版年：2000
• 期刊代码：34_03781097
• 类别：imm
• 出版时间：October 15, 2000
• 卷：191
• 期：2
• 页码：259-263
• 文件大小：268 K

摘要

An open reading frame of 885 nucleotides was identified as the Leptospira interrogans metF gene. The deduced amino acid sequence (294 amino acids) showed similarities with Escherichia coli methylene tetrahydrofolate reductase (MetF or MTHFR) (33%identity) and with the N-terminal part of human MTHFR (33%identity). The L. interrogans metF gene complements an E. coli metF mutant to prototrophy, suggesting the functionality of the folate branch converging to form methionine. In addition, the L. interrogans MetF was found to be thermolabile. The metF gene belonged to the CII secondary chromosome, in contrast to the previously isolated metY and metX genes, which have been localized to the CI chromosome of Leptospira sp.