Recombinant-α2b-interferon treatment in patients with chronic active hepatitis: effects on peripheral blood mononuclear cells and correlation with response
- 作者：De Lazzari ; Franca ; De Silvestro ; Giustina ; Floreani ; Annarosa ; Fabris ; Paolo ; Chiaramonte ; Maria ; et. al.
- 刊名：Current Therapeutic Research
- 出版年：1995
- 期刊代码：75_0011393x
- 类别：med
- 出版时间：April, 1995
- 卷：56
- 期：4
- 页码：419-428
- 文件大小：452 K
摘要
The purpose of this study was to evaluate the relationship between peripheral blood monocyte and lymphocyte subsets and response to recombinant-α2b-interferon (r-α2b-IFN) in patients with viral chronic active hepatitis (CAH). Peripheral blood mononuclear cell subsets were studied by use of flow cytometry before beginning treatment, after 24 hours, and after 15, 30, and 90 days of treatment with r-α2b-IFN (3 million U thrice weekly) in 16 patients with CAH—11 who tested anti-HCV—positive and five HBsAg-positive/anti-HBe—positive/HBV-DNA—positive (HCV = hepatitis C virus; HBsAg = hepatitis B surface antigen; HBe = hepatitis B e antigen; HBV = hepatitis B virus). r-α2b-IFN acutely decreased the number of peripheral blood B lymphocytes, natural killer (NK) cells, and T-naive cells. It also activated monocytes and T lymphocytes, as indicated by increased expression of surface human leukocyte antigen (HLA)-DR molecules and interleukin-2 receptors, respectively. These effects were no longer evident after 15 days of treatment. The number of NK cells was significantly lower in anti-HCV—positive than in HBsAg-positive patients (CD16+ cells: 277 ± 55/μL vs 450 ± 33/μL, P < 0.03) and was decreased in both groups during the 3 months of therapy. Responders to r-α2b-IFN did not differ from nonresponders except in the number of NK cells, which was higher than in anti-HCV—positive nonresponders 24 hours after the first injection. r-α2b-IFN acutely activates peripheral blood mononuclear cells and decreases the number of circulating NK cells in anti-HCV—positive and HBsAg-positive/anti-HBe—positive/HBV-DNA—positive patients with CAH. These effects did not correlate with treatment response.