Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating lFuc伪1鈫? dMan伪1鈫?and Gal尾1鈫?/4GlcNAc glycotopes

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• 作者：Albert M. Wu^a ; ^{amwu@mail.cgu.edu.tw} ; Jia-Hua Liu^a ; Anthony Herp^a ; Dvora Sudakevitz^b ; Nechama Gilboa-Garber^b
• 关键词：ABH active O-glycans ; Carbohydrate recognition domain ; Mannan ; Ralstonia solanacearum lectin
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：7 May, 2012
• 卷：586
• 期：9
• 页码：1294-1299
• 文件大小：402 K

摘要

Owing to the weak reactivities of monomeric dMan伪1 and Gal尾1鈫?/4GlcNAc尾 (ong class="boldFont">Iong>_尾/ong class="boldFont">IIong>_尾) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes lFuc伪1鈫? dMan伪1鈫?and ong class="boldFont">Iong>_尾/ong class="boldFont">IIong>_尾 within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of lFuc伪1鈫?complex enhanced the recognition intensities at lFuc伪1鈫?site, polyvalent dMan伪1鈫?was essential for binding at the dMan伪1鈫?site and polyvalent ong class="boldFont">Iong>_尾/ong class="boldFont">IIong>_尾 was required at lFuc伪1鈫?site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.