Structures of a unique O-polysaccharide of Edwardsiella tarda PCM 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing O-polysaccharide shared by E. tarda PCM 1145, PCM 1151 and PCM 1158

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• 作者：Ewa Katzenellenbogen^a ; ^{katzenel@iitd.pan.wroc.pl} ; ^{katzenel@immuno.iitd.pan.wroc.pl} ; Nina A. Kocharova^b ; Philip V. Toukach^b ; Sabina G&oacute ; rska^a ; Maria Bogulska^a ; Andrzej Gamian^a ; ^c ; Yuriy A. Knirel^b
• 关键词：Edwardsiella tarda ; Lipopolysaccharide ; O-Antigen ; Bacterial polysaccharide structure ; Abequose ; 2-Amino-2-deoxyglycerol
• 刊名：Carbohydrate Research
• 出版年：2012
• 期刊代码：15_00086215
• 类别：ch
• 出版时间：1 July, 2012
• 卷：355
• 期：Complete
• 页码：56-62
• 文件大小：711 K

摘要

Lipopolysaccharides of four strains of Edwardsiella tarda were degraded by mild acid hydrolysis, and the released O-polysaccharides were isolated by GPC and studied by sugar and methylation analyses along with ¹H and ¹³C NMR spectroscopy, including 2D ¹H, ¹H COSY, TOCSY, ROESY, ¹H, ¹³C HMBC, HSQC and HSQC-TOCSY experiments. The O-polysaccharide from E. tarda PCM 1153 was found to contain d-GalA, d-GlcNAc, d-Gal and 2-amino-1,3-propanediol (GroN). In the tetrasaccharide repeating unit, GroN is amide-linked to one of the GalA residues, and Gal is non-stoichiometrically 2- or 3-O-acetylated (鈭?5%at each position):

Three other E. tarda strains examined (PCM 1145, PCM 1151 and PCM 1158) share the following O-polysaccharide structure:

where Abe indicates 3,6-dideoxy-d-xylo-hexose (abequose). This structure resembles those of Citrobacter freundii O22 (PCM 1555) and Salmonella enterica O4. In accordance with the structural data, SDS-PAGE and immunoblotting of the lipopolysaccharides with anti-C. freundii O22 serum demonstrated that the O-antigens of the three E. tarda strains are serologically identical to each other and to the O-antigens of C. freundii O22 and S. enterica O4.