Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst

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• 作者：Kuan-Jung Chen^a ; Chia-Feng Lee^a ; John Rick^a ; Shih-Han Wang^b ; Chung-Chiun Liu^c ; Bing-Joe Hwang^a ; ^{bjh@mail.ntust.edu.tw}
• 关键词：Pt-based bimetallic nanoparticles (NPs) ; PtPd NPs-decorated multi walled carbon nanotube catalyst ; Glucose amperometric sensor ; Hydrogen peroxide sensing
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 March, 2012
• 卷：33
• 期：1
• 页码：75-81
• 文件大小：1044 K

摘要

A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Na铿乷n film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062-14.07 mM) and a low detection limit 0.031 mM. The biosensor also showed a short response time (within 5 s), and a high sensitivity (112 渭A mM^鈭? cm^鈭?). The Michaelis-Menten constant (K_m) was determined as 3.3 mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.