Kinetics of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle. Effect of agents possessing chaperone-like activity

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• 作者：Olga I. Maloletkina^a ; Kira A. Markossian^a ; Natalia A. Chebotareva^a ; Regina A. Asryants^b ; Sergey Yu. Kleymenov^a ; ^c ; Nikolay B. Poliansky^d ; Konstantin O. Muranov^d ; Valentina F. Makeeva^a ; Boris I. Kurganov^a ; ^{kurganov@inbi.ras.ru}
• 关键词：DLCA ; diffusion-limited cluster-cluster aggregation ; DLS ; dynamic light scattering ; DSC ; differential scanning calorimetry ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; HP-尾-CD ; 2-hydroxypropyl-尾-cyclodextrin ; Phb ; glycogen phosphorylase b ; RLCA ; re
• 刊名：Biophysical Chemistry
• 出版年：2012
• 期刊代码：11_03014622
• 类别：ch
• 出版时间：April, 2012
• 卷：163-164
• 期：Complete
• 页码：11-20
• 文件大小：943 K

摘要

An aggregation test system based on the aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle has been proposed. On the basis of the measurements of the enzyme activity and differential scanning calorimetry data a conclusion has been made that UV radiation results in formation of damaged protein molecules with lower thermostability. It was shown that the order of aggregation rate for UV-irradiated GAPDH with respect to the protein was close to 2. This means that such a test system allows detecting the effect of various agents exclusively on the stage of aggregation of unfolded protein molecules. The influence of 伪-crystallin and 2-hydroxypropyl-尾-cyclodextrin (HP-尾-CD) on aggregation of UV-irradiated GAPDH was studied. Despite the fact that HP-尾-CD accelerates thermal aggregation of non-irradiated GAPDH, in the case of aggregation of UV-irradiated GAPDH HP-尾-CD reveals a purely protective effect.