Trypanosoma rangeli expresses a 尾-galactofuranosyl transferase

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• 作者：Patrí ; cia Hermes Stoco^a ; ^{patriciastoco@gmail.com} ; Cassandra Aresi^a ; ^{cassandraaresi@hotmail.com} ; Dé ; bora Denardin Lü ; ckemeyer^a ; ^{debora_dl@hotmail.com} ; Maí ; sa Michels Sperandio^a ; ^{maisamichels@hotmail.com} ; Thaí ; s Cristine Marques Sincero^a ; ^{tsincero@ccs.ufsc.br} ; Má ; rio Steindel^a ; ^{mario.steindel@ufsc.br} ; Luiz Claudio Miletti^b ; ^{lcmilett@yahoo.com.br} ; Edmundo Carlos Grisard^a ; ^{edmundo.grisard@ufsc.br}
• 关键词：Trypanosoma rangeli ; GALFT ; Galactofuranosyl transferase ; Galactofuranose
• 刊名：Experimental Parasitology
• 出版年：2012
• 期刊代码：99_00144894
• 类别：imm
• 出版时间：March, 2012
• 卷：130
• 期：3
• 页码：246-252
• 文件大小：904 K

摘要

Glycoconjugates play essential roles in cell recognition, infectivity and survival of protozoan parasites within their insect vectors and mammalian hosts. 尾-galactofuranose is a component of several glycoconjugates in many organisms, including a variety of trypanosomatids, but is absent in mammalian and African trypanosomes. Herein, we describe the presence of a 尾(1-3) galactofuranosyl transferase (GALFT), an important enzyme of the galactofuranose biosynthetic pathway, in Trypanosoma rangeli. The T. rangeli GALFT gene (TrGALFT) has an ORF of 1.2 Kb and is organized in two copies in the T. rangeli genome. Antibodies raised against an internal fragment of the transferase demonstrated a 45 kDa protein coded by TrGALFT was localized in the whole cytoplasm, mainly in the Golgi apparatus and equally expressed in epimastigotes and trypomastigotes from T. rangeli. Despite the high sequence similarity with Trypanosoma cruzi and Leishmania spp. orthologous TrGALFT showed a substitution of the metal-binding DXD motif, conserved amongst glycosyltransferases, for a DXE functionally analogous motif. Moreover, a reduced number of GALFT genes were present in T. rangeli when compared with other pathogenic kinetoplastid species.